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Alliance Française contre les Maladies Parasitaires


heidelberg unilogo

PhD Position in Single Particle Imaging of P. falciparum Knobs

Project Leader

Prof. Dr. Michael Lanzer (Heidelberg University Hospital, Centre for Infectious Diseases, Parasitology Unit, INF 324, 69120 Heidelberg, Germany) www.ukhd.de/parasitologie

Project:

The objective is to provide a high-resolution map of the structure of P. falciparum knobs, respectively knob components, using biochemical approaches followed by single particle imaging (more details in attached PDF file)

Activities and experience:

Qualified candidates should have a Masters degree from an internationally accredited institution of higher education. Technical skills in biochemistry are desirable. Interest in interdisciplinary collaborations is mandatory. Applications from all fields of natural sciences are welcome as are candidates with a migration background. We also encourage scientists from Eastern European countries, including Ukraine and Russia to apply.

Application:

Online application : https://my-hbigs.uni-heidelberg.de/...

Deadline: June 30, 2022 | Start of PhD project: as early as September 2022

pdfPhD-Student_Lanzer_0122.pdf

ecpm 17 universite strasbourg 

PostDoc position in Biological Chemistry/Biochemistry/Proteomics at University of Strasbourg, France 

A renewable 6 month post-doctoral position is open at the ECPM (University of Strasbourg) to use activity-based protein profiling (ABPP)-based probes to evaluate the metabolism of the antiplasmodial drug plasmodione & derivatives, and to fish drug targets in living cells (E. coli, S, cerevisiae, P. falciparum). To circumvent the enhanced transmission of drug resistant malaria parasites to mosquitoes the team of the coordinator has developed an optimized plasmodione analogue with potent anti-gametocyte V and transmission-blocking properties. The postdoc project will use photoreactive plasmodione-derived alkynes as partners of the click reaction to fish and reveal target-protein adducts in mature P. falciparum rings and gametocytes upon irradiation and analyze them by mass spectrometry in chemical proteomics studies.

Project leader:

The project will be supervised by Dr. Elisabeth Davioud-Charvet, and integrated within a French/Swiss consortium working on novel antimalarial drugs and encompassing multiple disciplines: biological chemistry of redox-active antiparasitic compounds (Dr. Elisabeth Davioud-Charvet at LIMA-Strasbourg, & Marjorie Schmitt at LIMA-Mulhouse, UMR7042), mass spectrometry analysis & proteomics (Dr. Christine Schaeffer & Jean-Marc Strub) at IPHC, LSMBO, Strasbourg, Dr. Sarah Cianferani)), the biology of mosquitoes and malarial parasites (Dr Stephanie Blandin, MIR/M3I, IBMC, Strasbourg), and parasitology and drug screening (Prof. Pascal Maeser, University of Basel, Switzerland).

Project:

For the ROSkillers project, new selective activity-based probes were designed for the identification of plasmodione protein targets. A first proof-of-concept has been reported in vitro (Cichocki B. et al., JACSAu 2021, 1(5):669-689. doi: 10.1021/jacsau.1c00025). The postdoctoral researcher will undertake the analysis of the global interactome of this promising agent in vivo, first in E. coli and/or S. cerevisae overexpressing flavoenzymes as models, and then in parasite asexual stages and in gametocytes using proteomics methodologies. Candidate targets will be then validated with genetic engineering in Plasmodium. .  

Activities and experience:

We are looking for a highly motivated and ambitious candidate with a strong background in mass spectrometry analysis of proteins (fundamental criteria). Additional expertise in click chemistry and/or photoirradiation would be appreciated. The ideal candidate will have a proven track record of scientific excellence, very good communication and writing skills as well as a strong commitment to experimental work. The candidate should be also able to perform independent research and be eager to engage in collaborations with co-workers within this project. Knowledge of the French language is not required. Thorough knowledge of English, both oral and written is mandatory.. 

Application:

Application deadline : 30/04/2022

Application e-mail : christine.schaeffer (at) unistra.fr, sblandin (at) unistra.fr , elisabeth.davioud (at) unistra.fr

pdfJobOffer_post-doc_EUCOR2022_final.pdf

  08 universite bordeaux 

 

Intitulé du profil: 
Concours externe - Assistant ingénieur (ASI) en biologie, sciences de la vie et de la terre

Description:
Etude du cytosquelette chez les parasites Trypanosoma brucei et Toxoplasma gondii par des approches de biologie moléculaire et cellulaire et d’imagerie.

Structure de Recherche d'accueil:
Equipe « Protist Parasite Cytoskeleton » (Dr Mélanie Bonhivers & Dr Derrick Robinson) du département Microbiologie Fondamentale et Pathogénicité, UMR 5234, CNRS-Université de Bordeaux

Profil du candidat :

  • Connaissance générale en biologie cellulaire et moléculaire
  • Connaissance générale des appareillages spécifiques : microscopie de fluorescence, électrophorèses (ADN, protéines)
  • Connaissance des règles d'hygiène et de sécurité liées à la manipulation des produits et au travail en salle de culture de confinement L2
  • Bonne connaissance de la langue anglaise (écrit, parlé)
  • Maîtriser les techniques de la culture cellulaire (bactéries, levures, cellules mammifères), la culture de parasites est un plus.
  • Savoir travailler dans un module de recherche et en L2.
  • Savoir mettre en oeuvre les techniques de biologie moléculaire (clonage) et biologie cellulaire (immunomarquage, transfection)
  • Savoir mettre en oeuvre les techniques de microscopie à fluorescence
  • Savoir adapter des protocoles techniques en concertation avec les responsables de projet
  • Savoir rédiger des procédures techniques
  • Savoir assurer la traçabilité des données traitées et la reproductibilité des analyses réalisées
  • Savoir présenter les résultats par écrit et oralement de façon succincte et claire.
  • Savoir tenir à jour son cahier de laboratoire à jour.
  • Rigueur, éthique expérimentale
  • Sens de l'organisation, capacité à gérer plusieurs tâches en parallèle
  • Savoir travailler de façon autonome mais en interaction avec les autres membres du groupe (chercheurs, techniciens, étudiants, stagiaires) et de l’UMR.

Candidature :
Inscription sur Internet : http://www.enseignementsup-recherche.gouv.fr/recrutements/itrf du 31 mars 2022 au 28 avril 2022

Profil de poste :

https://www.u-bordeaux.fr/Universite/Recrutement/Concours-et-campagnes-de-recrutement/Concours-et-recrutements-ITRF-Session-2022
pdfFiche_poste_2022-ASI-Bap_A-SBM_MFP_UMR5234.pdf

06 Pasteur Logo Parafrap transparent 

PostDoc position in molecular biology at Institut Pasteur, Paris, France 

Lucy Glover's team is looking to recruit a highly motivated post-doctoral scientist to come work at the Institut Pasteur to study the role of specific post translational modifications on antigen gene switching in African trypanosomes https://research.pasteur.fr/en/team/trypanosomes-molecular-biology/.

Project leader:

Lucy Glover, Ph.D. Head, Trypanosome Molecular Biology group, Department of Parasites and Insect Vectors, Institut Pasteur Paris

Project:

Antigenic variation is a fundamental process used by pathogens to evade the host immune response. In T. brucei AV is the stochastic switching of the variant surface glycoprotein (VSG). It has been shown that inherent fragility within a VSG expression site (ES, locus of VSG gene expression) leads to the formation of double strand breaks (DSB) which triggers VSG recombination events and switching (Boothroyd, C, Nature, 2009; Glover, L, Plos Pathog, 2013). A repertoire of approximately 2000 VSG genes form a vast subtelomeric archive which enables the trypanosome to continuously switch VSGs and prolong an infection. This archive is accessed in a semi-predictable order during an infection. There are several outstanding questions to understanding AV in trypanosomes. The team is interested in what factors influence VSG recombination and VSG switching. The aim of this study is to dissect the effect of acetylation on the DSB repair (DSBR) and VSG recombination.  

Activities and experience:

The team is looking for a motivated post-doctoral researcher with a strong background in molecular biology. This project will involve molecular biology based techniques, RNA and DNA-seq, bioinformatic analysis of T. brucei. A background in parasitology is not essential for this position, but some experience in bioinformatics will be beneficial. The successful applicant will be expected to work independently, analysis and present their data within the lab and at international conferences. This project is funded by the ParaFrap LabEx

Application:

Please send your CV, three references and a motivation letter to lucy.glover (at sign) pasteur.fr  by the 31st of March 2022.

pdfParaFrap_Post-doctoral_position.pdf

20 INRAE  

Permanent position for an engineer in molecular biology (restricted to people who have a disability) 

An Engineer (“ingénieur d’étude”) permanent position is open in the lab in Tours for a molecular parasitologist. This position is restricted to people who have a disability.

If a putative candidate needs an advice regarding his/her eligibility to this position, he/she can contact evelyne.dupre (at sign) inrae.fr (human resources manager for the animal health division).

Projects leaders:

Contacts: Fabrice Laurent - fabrice.laurent (at sign) inrae.fr - & Julie Tottey - julie.tottey (at sign) inrae.fr

Lab:

The unit «Infectiology and Public Health» (1282 ISP) is a joined research unit hosting research teams from INRAE and the University of Tours. Within this unit, the team «Apicomplexa and Mucosal Immunity» (AIM) focuses on diseases caused by parasites multiplying in the digestive tract of livestock species and responsible for important economic losses: cryptosporidiosis in young ruminants and coccidiosis in poultry. With the aim to reduce the impact of these diseases, the team is developing approaches to enhance existing therapeutic options, or offer new alternative solutions taking societal demand into account. Cryptosporidium and Eimeria parasites have long been unresponsive to CRISPR-Cas9-mediated genetic modification but recent technological breakthrough in that field now allows (i) identification of important virulence factors (ii) identification of host response modulation (iii) vaccine production and (iv) validation of new molecular drug targets. 

Tasks and activities:

We are looking for a molecular biology engineer to join our dynamic team composed of 15 staff members including 5 researchers and 4 research technicians. You will play a key role in our team being the referent on parasite transgenesis and on the associated molecular engineering techniques. The engineer will support:
- monitoring of scientific literature on the development of transgenesis methods for Apicomplexa parasites.
- establish, develop and optimise autonomously innovative technological approaches in the field of transgenesis: loss and gain of function, inducible expression systems, stage-specific reporter gene expression, tagging... for Cryptosporidium and Eimeria.
- analyse -omics data (genomics, transcriptomics and proteomics) in interaction with the bioinformatics group hosted within the ISP research unit.
- exploit and analyse the results and data produced, and engage in their valorisation by participating in the writing of scientific publications.
- engage in activities related to the smooth running of the team: device and/or laboratory management and maintenance, protocol writing and update, and student training.
- comply with safety rules and regulation related to GMO handling.
- engage in technological projects set up together with the researchers in the team and in association with academic or private partners (French or international).
Your work will be associated to different research projects led by the researchers in the team.

Skills and experience:

- Scientific academic or engineering degree in biology with a very good experience in molecular biology.
- Ideally, the candidate will have experience in the genetic manipulation of apicomplexan parasites and on genome analysis.
- Very good knowledge of molecular approaches (ideally on parasites) and ability to analyse -mics data.
- Ability to innovate and monitor the scientific literature together with a strong affinity for new and innovative technologies.
- Rigour and organisation skills.
- Teamwork skills.
- Ability to interact with multiple collaborators (colleagues from the team, technological platforms, bioinformaticians, scientific collaborators).
- Very good English skills.

Details:

https://jobs.inrae.fr/handicap/campagne-handicap-ingenieurs-cadres-techniciens-h-f/ie22-sa-2