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French Alliance for Parasitology and Health Care

  17 universite strasbourg 

PostDoc position : Cross-talk between stress response and infection in dendritic cells at Institut Pasteur Lille, France 

We seek to hire a highly motivated candidate to work on the role of the Unfolded Protein Response (UPR) in modulating type 1 conventional dendritic cell (cDC1) functions in the context of Toxoplasma gondii infection. We recently unravelled that induction of the IRE1 pathway of the UPR is essential to activate key cDC1 functions and establish an efficient T-cell mediated control of parasite dissemination during acute toxoplasmosis (Poncet A et al. EMBO R, 2021, PMID: 33586853). We now aim to identify the involved molecular mechanisms, focusing on the cross-talk between changes in lipid metabolism induced by the infection and UPR-mediated regulation of cDC1 maturation and MHC-I antigen presentation. To this aim transgenic mice, in vitro cDC1 models and transgenic T. gondii strains will be used. The study will combine immuno-phenotyping techniques, metabolic assays, cellular biology and transcriptomic approaches.

The CIIL is a dynamic research center located in the Pasteur Institute of Lille that comprises internationally recognized groups focusing on immunity to infections and microbiology (https://ciil.fr/). The project is also embedded in a research consortium consisting of experts in UPR and Immunity (S. Janssens, IRC-VIB, Ghent) and in DC biology and T cell responses in the context of infections by intracellular parasites (N. Blanchard, Infinity, Toulouse). The candidate will be an active part of this consortium and will interact and collaborate with the other partners.

Highly motivated scientists with a strong interest in host-pathogen interactions are encouraged to apply. The candidate should have a PhD in Life Sciences with a strong knowledge in immunology. Experience with animal experimentation and flow cytometry is mandatory. Experience in imaging techniques would also be appreciated. Good writing and oral communication skills will be needed. The candidate should have demonstrated initiative, problem-solving attitude, and ability to work well both independently and as a team member.

CV, list of publications, short description of previous research projects and experience, and contact information for two referees should be sent by email to: sabrina.marion (at) pasteur-lille.fr.

pdfpost-doc_offer_Institut_Pasteur_Lille.pdf

  17 universite strasbourg 

PostDoc position in Biological Sciences / Biochemistry: Mode of action of antimalarial benzylmenadiones at IBMC, Strasbourg, France 

A renewable 1-year postdoctoral position is open at IBMC (Inserm/CNRS/University of Strasbourg) to investigate the mode of action of novel and promising antimalarial benzylmenadiones with potent curing and transmission blocking activities. This project is based on a combination of complementary strategies, notably genetics, activity-based protein profiling and proteomics in both yeast and Plasmodium parasites, to identify putative benzylmenadione targets. Candidate targets will be validated by genetic engineering in Plasmodium, and their role in parasite biology and in mediating benzylmenadione activity will be investigated.

Project leader:

The project will be supervised by Dr. Stephanie Blandin. It is part of a French/Swiss collaborative consortium working on novel antimalarial drugs and encompassing multiple disciplines: medicinal chemistry of redox-active antiparasitic compounds (Dr. Elisabeth Davioud-Charvet at ECPM, Strasbourg), mass spectrometry analysis & proteomics (Drs. Christine Schaeffer & Jean-Marc Strub at IPHC, Strasbourg), parasitology and drug screening (Profs. Pascal Maeser & Jennifer Keiser at Swiss TPH in Basel, Switzerland), yeast genetics (Dr. Brigitte Meunier at I2BC, Gif-sur-Yvette, France) and biology of mosquitoes and malarial parasites (Dr Stephanie Blandin at IBMC, Strasbourg).

Project:

The researcher will join the research unit Insect Models of Innate Immunity (M3i), located at the Institute of Molecular and Cellular Biology (IBMC) in Strasbourg, and more particularly the Inserm team 'Immune responses in mosquito vectors'. This group, led by Dr. Stephanie Blandin, is dedicated to the study of the resistance/susceptibility of mosquitoes to pathogens transmitted to humans (parasites and viruses), and to the development of novel strategies to limit disease transmission, including (but not limited to) novel drugs.

The postdoctoral researcher will start by evaluating a series of candidate target genes to decipher the mode of action of antimalarial benzylmenadiones in Plasmodium parasites. In parallel, he/she will look for additional candidates using an affinity-based protein profiling approach coupled with proteomics for which we have already reported a first proofof- concept (Cichocki B. et al., JACSAu 2021, 1(5):669-689. doi: 10.1021/jacsau.1c00025). The candidate should be ready to come up with new models, and to develop relevant assays to test them. Main activities: - Parasitology: Plasmodium falciparum culture (both asexual and gametocytes), parasite enrichment, transfection, assays to measure parasite sensitivity and resistance to drugs, establishment of new assays. - Molecular Biology: construction of plasmids for CRISPR/Cas9 and Selection-Linked Integration (SLI) to generate knockout, overexpression and allele replacement of putative target genes, validation of cassette insertion... - Affinity-Based Protein Profiling to identify new benzylmenadione targets in parasites - Reporting of results in electronic lab-book & lab meetings, attendance to conferences, writing manuscripts. - Supervision of students (recommended) 

Activities and experience:

PhD degree required

We are looking for a highly motivated, and creative candidate with a strong background in parasitology (fundamental criteria). Additional expertise in drug discovery, molecular biology and/or proteomics would be appreciated. The ideal candidate will have a proven track record of scientific excellence, very good communication and writing skills as well as a strong commitment to experimental work. The candidate should be also able to perform independent research and will be encouraged to make use of the expertise and facilities of partners whenever necessary. Knowledge of the French language is not required. Thorough knowledge of English, both oral and written, is mandatory. 

Application:

Application deadline : 27/01/2023

Application e-mail : bstevenin (at) unistra.fr

pdfCALL_FOR_POST-DOC_ROSaction2022.pdf

06 Pasteur

PostDoc position studying host-pathogen interactions at Institut Pasteur, Paris, France

A postdoc position is available at the Institut Pasteur in the signalling and host-parasite interactions group, headed by Najma Rachidi, to investigate the mechanisms by which the parasite modulates host cell signalling, through the study of Leishmania casein kinase 1.

Team:

The project will be developed under the supervision of Dr Najma Rachidi (https://research.pasteur.fr/en/member/najma-rachidi/), PI of the signalling and host-parasite interactions group at the Institut Pasteur in Paris. Institut Pasteur is an international and multi-disciplinary research centre with access to state-of-the-art technologies RNAseq, imaging, electron microscopy...

Project:

During infection, Leishmania parasites release effector molecules via extracellular vesicles to modify their host cell and insure their survival. We are studying one of these effectors, Leishmania Casein Kinase 1, a signalling protein kinase essential for intracellular parasite survival. Our in vitro data suggest that this kinase might fulfil a significant part of its function in the macrophage, by regulating processes such as trafficking, translation or apoptosis; processes also modulated during Leishmania infection. Our data suggests that Leishmania Casein Kinase 1 is an important player in host-parasite interactions as well as an important target for anti-leishmanial drug therapy. The project, part of an ANR, will combine genome editing, siRNA, cutting-edge microscopy and the development of an innovative method to follow the dynamic of infection and its signalling signature with the aim to identify the L-CK1.2 host substrates essential for Leishmania infection. For more details about our work, visit https://research.pasteur.fr/en/team/signalling-and-host-parasite-interactions/.

Candidate eligibility requirements:

We are looking for a motivated post-doctoral researcher with a strong background in cell culture as well as knockout or knockdown genes (CRISPR Cas9, siRNA and ShRNA) in mammalian cells (cell lines and primary cells). A background in parasitology is not required but would be a bonus. The candidate should be able to work in a multi-disciplinary environment, have a solid record of scientific achievement and a decent level in English. The contract is for two years but the applicant will be strongly encouraged to apply for external and/or internal fellowships.

Application:

Please send your Please send a cover letter, a CV with a publication list and three references to Najma Rachidi.
Applications should be sent before November 15th, with a starting date for the postdoc, beginning 2023.

pdfPostdoc_Pasteur_Rachidi.pdf

heidelberg unilogo  

PhD Position in Single Particle Imaging of P. falciparum Knobs

Project Leader

Prof. Dr. Michael Lanzer (Heidelberg University Hospital, Centre for Infectious Diseases, Parasitology Unit, INF 324, 69120 Heidelberg, Germany) www.ukhd.de/parasitologie

Project:

The objective is to provide a high-resolution map of the structure of P. falciparum knobs, respectively knob components, using biochemical approaches followed by single particle imaging (more details in attached PDF file)

Activities and experience:

Qualified candidates should have a Masters degree from an internationally accredited institution of higher education. Technical skills in biochemistry are desirable. Interest in interdisciplinary collaborations is mandatory. Applications from all fields of natural sciences are welcome as are candidates with a migration background. We also encourage scientists from Eastern European countries, including Ukraine and Russia to apply.

Application:

Online application : https://my-hbigs.uni-heidelberg.de/...

Deadline: June 30, 2022 | Start of PhD project: as early as September 2022

pdfPhD-Student_Lanzer_0122.pdf

ecpm 17 universite strasbourg 

PostDoc position in Biological Chemistry/Biochemistry/Proteomics at University of Strasbourg, France 

A renewable 6 month post-doctoral position is open at the ECPM (University of Strasbourg) to use activity-based protein profiling (ABPP)-based probes to evaluate the metabolism of the antiplasmodial drug plasmodione & derivatives, and to fish drug targets in living cells (E. coli, S, cerevisiae, P. falciparum). To circumvent the enhanced transmission of drug resistant malaria parasites to mosquitoes the team of the coordinator has developed an optimized plasmodione analogue with potent anti-gametocyte V and transmission-blocking properties. The postdoc project will use photoreactive plasmodione-derived alkynes as partners of the click reaction to fish and reveal target-protein adducts in mature P. falciparum rings and gametocytes upon irradiation and analyze them by mass spectrometry in chemical proteomics studies.

Project leader:

The project will be supervised by Dr. Elisabeth Davioud-Charvet, and integrated within a French/Swiss consortium working on novel antimalarial drugs and encompassing multiple disciplines: biological chemistry of redox-active antiparasitic compounds (Dr. Elisabeth Davioud-Charvet at LIMA-Strasbourg, & Marjorie Schmitt at LIMA-Mulhouse, UMR7042), mass spectrometry analysis & proteomics (Dr. Christine Schaeffer & Jean-Marc Strub) at IPHC, LSMBO, Strasbourg, Dr. Sarah Cianferani)), the biology of mosquitoes and malarial parasites (Dr Stephanie Blandin, MIR/M3I, IBMC, Strasbourg), and parasitology and drug screening (Prof. Pascal Maeser, University of Basel, Switzerland).

Project:

For the ROSkillers project, new selective activity-based probes were designed for the identification of plasmodione protein targets. A first proof-of-concept has been reported in vitro (Cichocki B. et al., JACSAu 2021, 1(5):669-689. doi: 10.1021/jacsau.1c00025). The postdoctoral researcher will undertake the analysis of the global interactome of this promising agent in vivo, first in E. coli and/or S. cerevisae overexpressing flavoenzymes as models, and then in parasite asexual stages and in gametocytes using proteomics methodologies. Candidate targets will be then validated with genetic engineering in Plasmodium. .  

Activities and experience:

We are looking for a highly motivated and ambitious candidate with a strong background in mass spectrometry analysis of proteins (fundamental criteria). Additional expertise in click chemistry and/or photoirradiation would be appreciated. The ideal candidate will have a proven track record of scientific excellence, very good communication and writing skills as well as a strong commitment to experimental work. The candidate should be also able to perform independent research and be eager to engage in collaborations with co-workers within this project. Knowledge of the French language is not required. Thorough knowledge of English, both oral and written is mandatory.. 

Application:

Application deadline : 30/04/2022

Application e-mail : christine.schaeffer (at) unistra.fr, sblandin (at) unistra.fr , elisabeth.davioud (at) unistra.fr

pdfJobOffer_post-doc_EUCOR2022_final.pdf

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