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French Parasitology Alliance For Health Care


06 Pasteur

PostDoc position studying host-pathogen interactions at Institut Pasteur, Paris, France 

A postdoc position is available at the Institut Pasteur in the signalling and host-parasite interactions group, headed by Najma Rachidi, to investigate the mechanisms by which the parasite modulates host cell signalling, through the study of Leishmania casein kinase 1.

Team:

The project will be developed under the supervision of Dr Najma Rachidi (https://research.pasteur.fr/en/member/najma-rachidi/), PI of the signalling and host-parasite interactions group at the Institut Pasteur in Paris. Institut Pasteur is an international and multi-disciplinary research centre with access to state-of-the-art technologies RNAseq, imaging, electron microscopy...

Project:

During infection, Leishmania parasites release effector molecules via extracellular vesicles to modify their host cell and insure their survival. We are studying one of these effectors, Leishmania Casein Kinase 1, a signalling protein kinase essential for intracellular parasite survival. Our in vitro data suggest that this kinase might fulfil a significant part of its function in the macrophage, by regulating processes such as trafficking, translation or apoptosis; processes also modulated during Leishmania infection. Our data suggests that Leishmania Casein Kinase 1 is an important player in host-parasite interactions as well as an important target for anti-leishmanial drug therapy. The project, part of an ANR, will combine genome editing, siRNA, cutting-edge microscopy and the development of an innovative method to follow the dynamic of infection and its signalling signature with the aim to identify the L-CK1.2 host substrates essential for Leishmania infection. For more details about our work, visit https://research.pasteur.fr/en/team/signalling-and-host-parasite-interactions/.  

Candidate eligibility requirements:

We are looking for a motivated post-doctoral researcher with a strong background in cell culture as well as knockout or knockdown genes (CRISPR Cas9, siRNA and ShRNA) in mammalian cells (cell lines and primary cells). A background in parasitology is not required but would be a bonus. The candidate should be able to work in a multi-disciplinary environment, have a solid record of scientific achievement and a decent level in English. The contract is for two years but the applicant will be strongly encouraged to apply for external and/or internal fellowships.

Application:

Please send your Please send a cover letter, a CV with a publication list and three references to Najma Rachidi.
Applications should be sent before November 15th, with a starting date for the postdoc, beginning 2023.

pdfPostdoc_Pasteur_Rachidi.pdf

heidelberg unilogo

PhD Position in Single Particle Imaging of P. falciparum Knobs

Project Leader

Prof. Dr. Michael Lanzer (Heidelberg University Hospital, Centre for Infectious Diseases, Parasitology Unit, INF 324, 69120 Heidelberg, Germany) www.ukhd.de/parasitologie

Project:

The objective is to provide a high-resolution map of the structure of P. falciparum knobs, respectively knob components, using biochemical approaches followed by single particle imaging (more details in attached PDF file)

Activities and experience:

Qualified candidates should have a Masters degree from an internationally accredited institution of higher education. Technical skills in biochemistry are desirable. Interest in interdisciplinary collaborations is mandatory. Applications from all fields of natural sciences are welcome as are candidates with a migration background. We also encourage scientists from Eastern European countries, including Ukraine and Russia to apply.

Application:

Online application : https://my-hbigs.uni-heidelberg.de/...

Deadline: June 30, 2022 | Start of PhD project: as early as September 2022

pdfPhD-Student_Lanzer_0122.pdf

ecpm 17 universite strasbourg 

PostDoc position in Biological Chemistry/Biochemistry/Proteomics at University of Strasbourg, France 

A renewable 6 month post-doctoral position is open at the ECPM (University of Strasbourg) to use activity-based protein profiling (ABPP)-based probes to evaluate the metabolism of the antiplasmodial drug plasmodione & derivatives, and to fish drug targets in living cells (E. coli, S, cerevisiae, P. falciparum). To circumvent the enhanced transmission of drug resistant malaria parasites to mosquitoes the team of the coordinator has developed an optimized plasmodione analogue with potent anti-gametocyte V and transmission-blocking properties. The postdoc project will use photoreactive plasmodione-derived alkynes as partners of the click reaction to fish and reveal target-protein adducts in mature P. falciparum rings and gametocytes upon irradiation and analyze them by mass spectrometry in chemical proteomics studies.

Project leader:

The project will be supervised by Dr. Elisabeth Davioud-Charvet, and integrated within a French/Swiss consortium working on novel antimalarial drugs and encompassing multiple disciplines: biological chemistry of redox-active antiparasitic compounds (Dr. Elisabeth Davioud-Charvet at LIMA-Strasbourg, & Marjorie Schmitt at LIMA-Mulhouse, UMR7042), mass spectrometry analysis & proteomics (Dr. Christine Schaeffer & Jean-Marc Strub) at IPHC, LSMBO, Strasbourg, Dr. Sarah Cianferani)), the biology of mosquitoes and malarial parasites (Dr Stephanie Blandin, MIR/M3I, IBMC, Strasbourg), and parasitology and drug screening (Prof. Pascal Maeser, University of Basel, Switzerland).

Project:

For the ROSkillers project, new selective activity-based probes were designed for the identification of plasmodione protein targets. A first proof-of-concept has been reported in vitro (Cichocki B. et al., JACSAu 2021, 1(5):669-689. doi: 10.1021/jacsau.1c00025). The postdoctoral researcher will undertake the analysis of the global interactome of this promising agent in vivo, first in E. coli and/or S. cerevisae overexpressing flavoenzymes as models, and then in parasite asexual stages and in gametocytes using proteomics methodologies. Candidate targets will be then validated with genetic engineering in Plasmodium. .  

Activities and experience:

We are looking for a highly motivated and ambitious candidate with a strong background in mass spectrometry analysis of proteins (fundamental criteria). Additional expertise in click chemistry and/or photoirradiation would be appreciated. The ideal candidate will have a proven track record of scientific excellence, very good communication and writing skills as well as a strong commitment to experimental work. The candidate should be also able to perform independent research and be eager to engage in collaborations with co-workers within this project. Knowledge of the French language is not required. Thorough knowledge of English, both oral and written is mandatory.. 

Application:

Application deadline : 30/04/2022

Application e-mail : christine.schaeffer (at) unistra.fr, sblandin (at) unistra.fr , elisabeth.davioud (at) unistra.fr

pdfJobOffer_post-doc_EUCOR2022_final.pdf

  08 universite bordeaux 

 

Position: 
External Recruitment Competition (permanent position) - Assistant Engineer in biology, life and earth sciences

Details:
Study of the cytoskeleton in Trypanosoma brucei and Toxoplasma gondii parasites using molecular and cell biology and imaging approaches.

Laboratory:
Team " Protist Parasite Cytoskeleton " (Dr Mélanie Bonhivers & Dr Derrick Robinson) of the Department of Fundamental Microbiology and Pathogenicity, UMR 5234, CNRS-Université de Bordeaux

Skills and experience:

  • General knowledge of cellular and molecular biology
  • General knowledge of specific equipment: fluorescence microscopy, electrophoresis (DNA, proteins)
  • Knowledge of hygiene and safety rules related to the handling of products and work in the L2 containment culture room
  • Good knowledge of the English language (written, spoken)
  • Knowledge of cell culture techniques (bacteria, yeast, mammalian cells), parasite culture is a plus
  • Ability to work in a research module and in L2.
  • Know how to use molecular biology techniques (cloning) and cell biology (immunostaining, transfection)
  • Know how to use fluorescence microscopy techniques
  • Know how to adapt technical protocols in consultation with project managers
  • Know how to write technical procedures
  • Know how to ensure the traceability of the processed data and the reproducibility of the analyses performed
  • Know how to present the results in writing and orally in a clear and succinct manner.
  • Know how to keep his/her laboratory notebook up to date.
  • Rigor, experimental ethics
  • Organizational skills, ability to manage several tasks in parallel
  • Ability to work independently but in interaction with other members of the group (researchers, technicians, students, trainees) and of the department

Application :
Online : http://www.enseignementsup-recherche.gouv.fr/recrutements/itrf from 31 March 2022 to 28 April 2022

Job profile (in French):

https://www.u-bordeaux.fr/Universite/Recrutement/Concours-et-campagnes-de-recrutement/Concours-et-recrutements-ITRF-Session-2022
pdfFiche_poste_2022-ASI-Bap_A-SBM_MFP_UMR5234.pdf

20 INRAE  

Permanent position for an engineer in molecular biology (restricted to people who have a disability) 

An Engineer (“ingénieur d’étude”) permanent position is open in the lab in Tours for a molecular parasitologist. This position is restricted to people who have a disability.

If a putative candidate needs an advice regarding his/her eligibility to this position, he/she can contact evelyne.dupre (at sign) inrae.fr (human resources manager for the animal health division).

Projects leaders:

Contacts: Fabrice Laurent - fabrice.laurent (at sign) inrae.fr - & Julie Tottey - julie.tottey (at sign) inrae.fr

Lab:

The unit «Infectiology and Public Health» (1282 ISP) is a joined research unit hosting research teams from INRAE and the University of Tours. Within this unit, the team «Apicomplexa and Mucosal Immunity» (AIM) focuses on diseases caused by parasites multiplying in the digestive tract of livestock species and responsible for important economic losses: cryptosporidiosis in young ruminants and coccidiosis in poultry. With the aim to reduce the impact of these diseases, the team is developing approaches to enhance existing therapeutic options, or offer new alternative solutions taking societal demand into account. Cryptosporidium and Eimeria parasites have long been unresponsive to CRISPR-Cas9-mediated genetic modification but recent technological breakthrough in that field now allows (i) identification of important virulence factors (ii) identification of host response modulation (iii) vaccine production and (iv) validation of new molecular drug targets. 

Tasks and activities:

We are looking for a molecular biology engineer to join our dynamic team composed of 15 staff members including 5 researchers and 4 research technicians. You will play a key role in our team being the referent on parasite transgenesis and on the associated molecular engineering techniques. The engineer will support:
- monitoring of scientific literature on the development of transgenesis methods for Apicomplexa parasites.
- establish, develop and optimise autonomously innovative technological approaches in the field of transgenesis: loss and gain of function, inducible expression systems, stage-specific reporter gene expression, tagging... for Cryptosporidium and Eimeria.
- analyse -omics data (genomics, transcriptomics and proteomics) in interaction with the bioinformatics group hosted within the ISP research unit.
- exploit and analyse the results and data produced, and engage in their valorisation by participating in the writing of scientific publications.
- engage in activities related to the smooth running of the team: device and/or laboratory management and maintenance, protocol writing and update, and student training.
- comply with safety rules and regulation related to GMO handling.
- engage in technological projects set up together with the researchers in the team and in association with academic or private partners (French or international).
Your work will be associated to different research projects led by the researchers in the team.

Skills and experience:

- Scientific academic or engineering degree in biology with a very good experience in molecular biology.
- Ideally, the candidate will have experience in the genetic manipulation of apicomplexan parasites and on genome analysis.
- Very good knowledge of molecular approaches (ideally on parasites) and ability to analyse -mics data.
- Ability to innovate and monitor the scientific literature together with a strong affinity for new and innovative technologies.
- Rigour and organisation skills.
- Teamwork skills.
- Ability to interact with multiple collaborators (colleagues from the team, technological platforms, bioinformaticians, scientific collaborators).
- Very good English skills.

Details:

https://jobs.inrae.fr/handicap/campagne-handicap-ingenieurs-cadres-techniciens-h-f/ie22-sa-2