Lucy GLOVER |
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Information |
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Trypanosome Molecular Biology (TMB) | ||
Paris, Institut Pasteur | ||
0000-0001-7191-6890 | ||
This email address is being protected from spambots. You need JavaScript enabled to view it. | ||
https://research.pasteur.fr/en/team/trypanosomes-molecular-biology/ | ||
@Glover_Tryplab | ||
Scientific interests and projects |
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We research the host-pathogen relationship through understanding the mechanisms of DNA repair and allelic exclusion. Antigenic variation and immune evasion requires the DNA repair pathway and the strict monoallelic expression of a variant surface glycoprotein gene (VSG). To study the role of the DNA repair in antigenic variation, we use the I-SceI meganuclease system in Trypanosoma brucei (T. brucei) to temporally and spatially induce a single double-strand break (DSB) and study the dynamics of DSB repair and recombination. Our work has shown that the position of the DSB influences antigenic variation and repair pathway choice. Additionally, we use high-throughput functional RNAi librarys in trypanosomes to screen for factors required for VSG monoallelic control. This led to the identification of VEX1 (VSG EXclusion 1; Tb927.11.16920), a protein that controls monoallelic VSG gene expression. Within the framework of ParaFrap our work is aimed at decoding the genetic basis for repair and recombination in T. brucei and identify cohorts of genes required for antigenic variation. By exploiting both a proteomics (SILAC and IP) and genomics (forward genetic screens) approach our goal is to determine the composition of the DNA repair factors that are essential for antigenic variation. We also aim understand the trypanosomes mechanisms of pathogenesis by understanding the role of VEX1 in controlling Metacyclic VSG expression in the Tsetse fly insect vector. Only a single mVSG is expressed in each cell, but the mechanism by which this expression is regulated and the factors required are unknown. |
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Top 5 publications of the last 5 years |
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1. Faria, J., Glover, L., Hutchinson, S. et al. Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex. Nat Commun 10, 3023 (2019) doi: 10.1038/s41467-019-10823-8 2. Glover L, Marques CA, Suska O, Horn D. 2019. Persistent DNA damage foci and DNA replication with a broken chromosome in the African trypanosome. mBio 10:e01252-19. doi: 10.1128/mBio.01252-19 3. Hutchinson S, Glover L, Horn D. High-resolution analysis of multi-copy variant surface glycoprotein gene expression sites in African trypanosomes. BMC Genomics. 2016;17(1):806. Epub 2016/10/21. PubMed PMID: 27756224; PMCID: PMC5070307. doi: 10.1186/s12864-016-3154-8. 4. Glover L, Hutchinson S, Alsford S, Horn D. VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes. Proc Natl Acad Sci U S A. 2016;113(26):7225-30. Epub 2016/05/27. PubMed PMID: 27226299; PMCID: PMC4932947. doi: 10.1073/pnas.1600344113. 5. Glover L, Alsford S, Baker N, Turner DJ, Sanchez-Flores A, Hutchinson S, Hertz-Fowler C, Berriman M, Horn D. Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes. Nat Protoc. 2015;10(1):106-33. PubMed PMID: 25502887. doi: 10.1038/nprot.2015.005. |
Postdoctoral position, Institut Pasteur, Paris, France A 24-month post-doctoral position starting on March 1st 2025 and funded by the French National Research Agency (ANR) is available in the Trypanosome...
Postdoctoral positions, LPHI Lab, Montpellier, France Two ERC-funded (JANUS 2024-2029) postdoc positions are open in the lab to study cell cycle regulation in malaria...
Postdoctoral position, Institut Pasteur, Paris, France A postdoc position is available at the Institut Pasteur Paris, in the signalling and host-parasite interactions research group, headed by Dr Najma Rachidi...
ABOUT SPEAKERS PROGRAMME REGISTRATION CONTACT LOCATION About the workshop ParaFrap is organizing the ParaFrap Next Generation in Parasitology...
[Communiqué] As part of the actions of the Health Innovation Plan 2030, the health component of the France 2030 plan, Mohamed-Ali Hakimi is one of the 22 laureates of an Excellence Chair in Biology/Health, supported to conduct high-level research...
The York Biomedical Research Institute (YBRI) are hosting an online live seminar on Friday 24th May 1-2pm BST as part of their biomedical science seminar series. You are all welcome to attend - registration is required via the...
[Communiqué] Congratulations to Arthur Talman, IRD Research Leader in the MIVEGEC laboratory (Montpellier), who has been awarded an Advanced Grant from the European Research Council (ERC) for the project "TROJAN - Molecular mimicry and immune...
Ana Rita Gomes, a CNRS Research Associate at the Laboratory of Pathogens and Host Immunity in Montpellier, and former Postdoctoral fellow of the LabEx ParaFrap, has been awarded the prestigious CNRS Bronze Medal in 2023. This...
Happy New Year 2024! To kick off this new scientific year, here is the ParaFrap webinar schedule. Since January 2021, ParaFrap has been organizing a series of webinars. This monthly event, held every 2nd Thursday of the month, aims to strengthen...
The team of Dr. Mohamed-Ali Hakimi, in collaboration with Isabelle Coppens (Johns Hopkins University Bloomberg School of Public Health and Malaria Research Institute, Baltimore, MD, USA), has developed a new (epi)genetic method to produce in vitro...
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