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Chetan CHITNIS |
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Information |
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Institut Pasteur, Department of Parasites and Insect Vectors | |
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Malaria Parasite Biology and Vaccines | |
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Paris | |
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0000-0002-8773-9865 | |
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This email address is being protected from spambots. You need JavaScript enabled to view it. | |
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https://research.pasteur.fr/en/team/malaria-parasite-biology-and-vaccines/ | |
Scientific interests and projects |
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Our laboratory is interested in understanding the biology of blood stage malaria parasites. In particular we are interested in understanding the molecular processes that mediate merozoite egress from schizonts and merozoite invasion of RBCs. We study receptor-ligand interactions that mediate RBC invasion and the signaling processes that regulate steps such as microeneme and rhoptry secretion that are critical for receptor engagement during invasion. Information on parasite proteins involved in invasion will be used to develop multivalent blood stage vaccines for malaria. In the ParaFrap project, we will explore the signaling mechanisms involved in apical organelle secretion during host cell invasion. In particular we will study the role of second messengers including calcium and cyclic nucleotides, cAMP and cGMP, in regulating microneme secretion. A combination of molecular genetics and phosphoproteomic approaches will be used to decipher the signaling pathways responsible for microneme and rhoptry secretion. In addition, we will identify the critical parasite ligands involved in RBC invasion and try to identify combinations of parasite ligands that can be targeted with antibodies to synergistically block invasion with high efficiency. Such combinations will be developed as blood stage vaccines for P. falciparum malaria. Similar approaches will also be attempted with P. vivax for which short term in vitro invasion assays will be established using enriched reticulocytes and cryopresrved blood stage P. vivax isolates. |
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Top 5 publications of the last 5 years |
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1. Singh P., Alaganan A., More K. R., Lorthiois, A, Thiberge S Gorgette O., Guillotte Blisnick M., Guglielmini J., Aguilera S. S., Touqui L., Singh S., Chitnis CE. 2019. Role of a patatinlike phospholipase in Plasmodium falciparum gametogenesis and malaria transmission. Proc. Natl. Acad. Sci., USA. DOI 10.1073/pnas.1900266116. 2. Singh K, Mukherjee P, Shakri AR, Singh A, Pandey GC, Bakshi M, Uppal G, Jena R, Rawat A, Kumar P, Bhardwaj R, Yazdani SS, Hans D, Mehta S, Srinivasan A, Anil K, Madhusudhan RL Patel J, Singh A, Rao R, Gangireddy S, Patil R, Kaviraj S, Singh S, Carter D, Reed S, Kaslow DC, Birkett A, Chauhan VS, Chitnis CE. 2018. A malaria vaccine candidate based on Duffy binding protein elicits strain transcending functional antibodies in a Phase I clinical trial. NPJ Vaccines. 3:48. DOI 10.1038/s41541-018-0083-3. 3. Gupta S, Singh S, Popovici J, Roesch C, Shakri AR, Guillotte-Blisnick M, Huon C, Menard D, Chitnis CE. 2018. Targeting a reticulocyte binding protein and Duffy binding protein to inhibit reticulocyte invasion by Plasmodium vivax. Sci Rep. 8(1):10511. DOI 10.1038/s41598-018-28757-4. 4. Chitnis CE, Mukherjee P, Mehta S, Yazdani SS, Dhawan S, Shakri AR, Bhardwaj R, Gupta PK, Hans D, Mazumdar S, Singh B, Kumar S, Pandey G, Parulekar V, Imbault N, Shivyogi P, Godbole G, Mohan K, Leroy O, Singh K, Chauhan VS. 2015. Phase I clinical trial of a recombinant blood stage vaccine candidate for Plasmodium falciparum malaria based on MSP1 and EBA175. PLoS One. 10(4):e0117820. DOI doi.org/10.1371/journal.pone.0117820 5. Dawn A, Singh S, More KR, Siddiqui FA, Pachikara N, Ramdani G, Langsley G, Chitnis CE. The central role of cAMP in regulating Plasmodium falciparum merozoite invasion of human erythrocytes. 2014. PLoS Pathogens. 10(12):e1004520. DOI doi.org/10.1371/journal.ppat.1004520 |
[Publication] Les derniers travaux de Rania Najm, Hiba El Hajj, Maryse Lebrun & leurs collègues sont publiés dans PNAS. Les auteurs apportent des connaissances importantes sur les acteurs moléculaires de l'invasion des bradyzoïtes et...
LabEx ParaFrap wishes you happiness, health, science and success for 2023. First news from the network this year: the ParaFrap webinars will be back in 2023, as in previous years, every second Thursday of the month at 3pm. If you are not yet on the...
PostDoc position in Biological Sciences / Biochemistry: Mode of action of antimalarial benzylmenadiones at IBMC, Strasbourg, France A renewable 1-year postdoctoral position is open at IBMC (Inserm/CNRS/University...
[Communiqué] Gerald Spaeth, head of the "Molecular Parasitology and Signalling" unit at the Institut Pasteur Paris, has been awarded a Synergy grant from the European Research Council (ERC) for the project "DECOLeishRN - Decoding...
PostDoc position studying host-pathogen interactions at Institut Pasteur, Paris, France A postdoc position is available at the Institut Pasteur in the signalling and host-parasite interactions group, headed by Najma Rachidi, to investigate the...
[Communiqué] The team of Dr Chetan Chitnis from the Institut Pasteur, a partner in LabEx ParaFrap, has studied the blood stage of the Plasmodium falciparum parasite, which causes the most severe and deadly form of malaria, and discovered a...
PhD Position in Single Particle Imaging of P. falciparum Knobs Project Leader Prof. Dr. Michael Lanzer (Heidelberg University Hospital, Centre for Infectious Diseases, Parasitology Unit, INF 324, 69120 Heidelberg, Germany)...
PostDoc position in Biological Chemistry/Biochemistry/Proteomics at University of Strasbourg, France A renewable 6 month post-doctoral position is open at the ECPM (University of Strasbourg) to use activity-based protein...
Dear colleagues,The EMBO Workshop "New frontiers in host-parasite interactions, from cell to organism", supported by...
Position: External Recruitment Competition (permanent position) - Assistant Engineer in biology, life and earth sciences Details:Study of the cytoskeleton in Trypanosoma brucei and Toxoplasma gondii...
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