French Alliance for Parasitology and Health Care

  08 universite bordeaux 


External Recruitment Competition (permanent position) - Assistant Engineer in biology, life and earth sciences

Study of the cytoskeleton in Trypanosoma brucei and Toxoplasma gondii parasites using molecular and cell biology and imaging approaches.

Team " Protist Parasite Cytoskeleton " (Dr Mélanie Bonhivers & Dr Derrick Robinson) of the Department of Fundamental Microbiology and Pathogenicity, UMR 5234, CNRS-Université de Bordeaux

Skills and experience:

  • General knowledge of cellular and molecular biology
  • General knowledge of specific equipment: fluorescence microscopy, electrophoresis (DNA, proteins)
  • Knowledge of hygiene and safety rules related to the handling of products and work in the L2 containment culture room
  • Good knowledge of the English language (written, spoken)
  • Knowledge of cell culture techniques (bacteria, yeast, mammalian cells), parasite culture is a plus
  • Ability to work in a research module and in L2.
  • Know how to use molecular biology techniques (cloning) and cell biology (immunostaining, transfection)
  • Know how to use fluorescence microscopy techniques
  • Know how to adapt technical protocols in consultation with project managers
  • Know how to write technical procedures
  • Know how to ensure the traceability of the processed data and the reproducibility of the analyses performed
  • Know how to present the results in writing and orally in a clear and succinct manner.
  • Know how to keep his/her laboratory notebook up to date.
  • Rigor, experimental ethics
  • Organizational skills, ability to manage several tasks in parallel
  • Ability to work independently but in interaction with other members of the group (researchers, technicians, students, trainees) and of the department

Application :
Online : from 31 March 2022 to 28 April 2022

Job profile (in French):

20 INRAE  

Permanent position for an engineer in molecular biology (restricted to people who have a disability) 

An Engineer (“ingénieur d’étude”) permanent position is open in the lab in Tours for a molecular parasitologist. This position is restricted to people who have a disability.

If a putative candidate needs an advice regarding his/her eligibility to this position, he/she can contact evelyne.dupre (at sign) (human resources manager for the animal health division).

Projects leaders:

Contacts: Fabrice Laurent - fabrice.laurent (at sign) - & Julie Tottey - julie.tottey (at sign)


The unit «Infectiology and Public Health» (1282 ISP) is a joined research unit hosting research teams from INRAE and the University of Tours. Within this unit, the team «Apicomplexa and Mucosal Immunity» (AIM) focuses on diseases caused by parasites multiplying in the digestive tract of livestock species and responsible for important economic losses: cryptosporidiosis in young ruminants and coccidiosis in poultry. With the aim to reduce the impact of these diseases, the team is developing approaches to enhance existing therapeutic options, or offer new alternative solutions taking societal demand into account. Cryptosporidium and Eimeria parasites have long been unresponsive to CRISPR-Cas9-mediated genetic modification but recent technological breakthrough in that field now allows (i) identification of important virulence factors (ii) identification of host response modulation (iii) vaccine production and (iv) validation of new molecular drug targets. 

Tasks and activities:

We are looking for a molecular biology engineer to join our dynamic team composed of 15 staff members including 5 researchers and 4 research technicians. You will play a key role in our team being the referent on parasite transgenesis and on the associated molecular engineering techniques. The engineer will support:
- monitoring of scientific literature on the development of transgenesis methods for Apicomplexa parasites.
- establish, develop and optimise autonomously innovative technological approaches in the field of transgenesis: loss and gain of function, inducible expression systems, stage-specific reporter gene expression, tagging... for Cryptosporidium and Eimeria.
- analyse -omics data (genomics, transcriptomics and proteomics) in interaction with the bioinformatics group hosted within the ISP research unit.
- exploit and analyse the results and data produced, and engage in their valorisation by participating in the writing of scientific publications.
- engage in activities related to the smooth running of the team: device and/or laboratory management and maintenance, protocol writing and update, and student training.
- comply with safety rules and regulation related to GMO handling.
- engage in technological projects set up together with the researchers in the team and in association with academic or private partners (French or international).
Your work will be associated to different research projects led by the researchers in the team.

Skills and experience:

- Scientific academic or engineering degree in biology with a very good experience in molecular biology.
- Ideally, the candidate will have experience in the genetic manipulation of apicomplexan parasites and on genome analysis.
- Very good knowledge of molecular approaches (ideally on parasites) and ability to analyse -mics data.
- Ability to innovate and monitor the scientific literature together with a strong affinity for new and innovative technologies.
- Rigour and organisation skills.
- Teamwork skills.
- Ability to interact with multiple collaborators (colleagues from the team, technological platforms, bioinformaticians, scientific collaborators).
- Very good English skills.


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